siRNA / RNAi /miRNA transfection Human THP-1 cells

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A-172

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using in situ Cell Death Detection Kit, POD to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using EBMTM Endothelial Cell Growth Basal Medium, 500 mL to perform 3D Cell Culture Media Human blood-brain barrier organoid

Get tips on using Anti-Collagen Type VII Antibody, clone 32,-VII to perform Immunohistochemistry Collagen VII [II-32] - Mouse Human -NA-

Get tips on using Senescence Detection Kit - Merck to perform Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue TGFBI

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation SH-SY5Y ALK