Site Directed Mutagenesis (SDM) Human Point mutation HeLa

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Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized HeLa

Products Sigma-Aldrich GenElute™ Mammalian Total RNA Miniprep Kit

Get tips on using 24-Well Cell Invasion Assays, Basement Membrane to perform Cell migration / Invasion cell type - HeLa

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Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - HeLa cells

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Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - HeLa

Products Enzo Life Sciences ROS-ID® Total ROS/Superoxide detection kit

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

RNA RNA isolation / purification Bacteria Gram negative Helicobacter pylori

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Products Polyplus transfections jetPEI® DNA transfection, HTS application

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa

Products Promega Wizard® Genomic DNA Purification Kit

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - HeLa

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Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - HeLa

Products Cell Biolabs OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

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