ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
Get tips on using RevertAid RT Reverse Transcription Kit to perform
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using Omniscript RT Kit (200) to perform cDNA synthesis Cell lines
Get tips on using Sensiscript RT Kit (200) to perform cDNA synthesis Cell lines
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized 293T
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized VCaP
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U87
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized T98G
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized PNT2
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