Mammalian cell culture media

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The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

RNA siRNA / RNAi /miRNA transfection Rat IEC Cationic lipid based
Necrosis HUVEC Experiment

Cellular assays Necrosis HUVEC
Necrosis LNCaP Experiment

Cellular assays Necrosis LNCaP
Ne Caco-2 Experiment

Cellular assays Ne Caco-2
Necrosis HuH-7 Experiment

Cellular assays Necrosis HuH-7
Necrosis PC-3 Experiment

Cellular assays Necrosis PC-3

Cellular assays Purification of extracellular vesicles Exosomes Plasma
pJAP2 Product

Get tips on using pJAP2 to perform Protein Expression Eukaryotic cells - HEK293 AT1R

Products Christopher G. Tate, MRC Laboratory of Molecular Biology, Cambri pJAP2
pJAP34 Product

Get tips on using pJAP34 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Products Christopher G. Tate, MRC Laboratory of Molecular Biology, Cambri pJAP34
pJAP37 Product

Get tips on using pJAP37 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Products Christopher G. Tate, MRC Laboratory of Molecular Biology, Cambri pJAP37

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