Get tips on using Superscript reverse tran-scriptase II (SS RT II) system to perform cDNA synthesis Yeast
Get tips on using BH100bp ladder :: GD 100bp DNA Ladder H3 RTU Ladder to perform DNA Ladder 100 bp
Get tips on using QIAGEN OneStep Ahead RT-PCR Kit (200) to perform PCR Quantitative real-time PCR - Fish species DNA
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using QuikChange Lightning Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion L929 ICP6
Get tips on using LC3B antibody to perform Autophagy assay cell type - RT4
Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression HBV RT
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human RT-7
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