siRNA / miRNA gene silencing Human CRL-5915

Get tips on using pET-28a-Cramoll to perform Protein Expression Prokaryotic cells - E. coli rCramoll

Get tips on using TaqMan™ Cronobacter sakazakii Detection Kit to perform Cell Culture Contamination Detection Kit Bacteria

Get tips on using COLUMBIA BLOOD AGAR BASE to perform Bacterial cell culture media Clostridium difficile

Get tips on using pEThCRM to perform Protein Expression Prokaryotic cells - E. coli CRM197

Get tips on using pCRM197 to perform Protein Expression Prokaryotic cells - E. coli CRM197

Get tips on using pCcrtB to perform Protein Expression Prokaryotic cells - E. coli Kocuria gwangalliensis CrtB

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Cultrex® Collagen IV Cell Invasion Assay to perform Cell migration / Invasion cell type - TPC1

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.