RNA isolation / purification Tissue Rat

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

Get tips on using Unstained Protein Ladder, Broad Range Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained High Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Broad Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Low Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using Spectra™ Multicolor High Range Protein Ladder to perform Protein Ladder Prestained

Get tips on using Spectra™ Multicolor Low Range Protein Ladder to perform Protein Ladder Prestained

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Unstained Protein Standard, Broad Range (10-200 kDa) to perform Protein Ladder Unstained

Get tips on using Color Prestained Protein Standard, Broad Range (10-250 kDa) to perform Protein Ladder Prestained