DNA methylation profiling Gene specific profiling Rat whole pituitary glands

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using lentiCRISPR to perform CRISPR Mouse - Deletion RAW 264.7 Tfeb

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Raji

Get tips on using LC3B Antibody to perform Autophagy assay cell type - RAW 264.7

Get tips on using CAG-Cas9 to perform CRISPR Mouse - Deletion Neuro 2a Rab38

Get tips on using lentiCas9-Blast to perform CRISPR Mouse - Deletion RAW 264.7 Casp1

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Raji

Get tips on using TruSeq Stranded Total RNA to perform RNA sequencing Mouse - RAW264.7

Get tips on using ATG12 Antibody (166) to perform Autophagy assay cell type - RAW 264.7