siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - THP 1

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis PANC-1

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - SiHa TIMP2

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - HeLa TIMP2

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized Sf9

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized PNT2

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized HaCaT