Protein Expression

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Get tips on using p83Xi to perform Protein Expression Eukaryotic cells - S. cerevisiae Integral membrane proteins (IMPs)

Get tips on using pET28a-FSHR to perform Protein Expression Prokaryotic cells - E. coli FSHR-57aa proteins

Get tips on using TAGZyme pQE Vector Set to perform Protein tag Expression of His-tagged proteins

Get tips on using pLsLF-CTB-Fx3Pris to perform Protein Expression Eukaryotic cells - N. benthamiana proinsulin

Get tips on using pLDutr-CTB-Fx3Pris to perform Protein Expression Eukaryotic cells - lettuce leaves proinsulin

Get tips on using pDG101 to perform Protein Expression Prokaryotic cells - E. coli mScarlet-I

Get tips on using pZH713 to perform Protein Expression Prokaryotic cells - E. coli PP7cp-SYFP2

Get tips on using pFR2 to perform Protein Expression Prokaryotic cells - E. coli ferulic acid