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|To analyze gene expression of each cochlea turn, 12 SurePrint G3 Mouse Exon Microarrays (Agilent Technologies), which were spotted with 165,984 exon probes (24,547 genes), were hybridized to labeled cRNA (4 microarrays were used for each turn sample). Prior to the hybridization step, Cyanine 3-labeled cRNAs were fragmented using 25X fragmentation buffer at 60°C in a water bath for 30 min and then hybridized to a microarray slide for 17 hours at 65°C in a hybridization oven and washed using Gene Expression Wash Buffer (Agilent Technologies).
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