ChIP H3K27me3 Mouse Canine

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Purified anti-mouse TCR γ/δ Antibody to perform Flow cytometry Anti-bodies Mouse - TCRγ/δ

Get tips on using Biotin Rat Anti-Mouse OX40 Ligand (CD252) to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L

Get tips on using FITC anti-human/mouse Granzyme B Antibody to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Get tips on using PE-CF594 Rat Anti-Mouse Siglec-F to perform Flow cytometry Anti-bodies Mouse - Siglec F

Get tips on using FITC anti-mouse CD326 (Ep-CAM) Antibody to perform Flow cytometry Anti-bodies Mouse - CD326/EpCAM

Get tips on using ON-TARGETplus Mouse Stat5b (20851) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - FL83B Stat5b

Get tips on using ON-TARGETplus Mouse Cdh5 (12562) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MS1 Cdh5

Get tips on using ON-TARGETplus Mouse Cd84 (12523) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - M210B4 Cd84

Get tips on using ON-TARGETplus Mouse Tead4 (21679) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - C2C12 Tead4