PCR Quantitative real-time PCR

Get tips on using Mouse Prolactin ELISA Kit (ab100736) to perform ELISA Mouse - PRL

Get tips on using RIPA Lysis Buffer (ProteinSimple) to perform Protein isolation Tissue - Mouse aorta

Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Bordetella pertussis

Get tips on using pUC19/Pr-GLuc to perform Protein Expression Eukaryotic cells - K. lactis Gaussia luciferase

Get tips on using Qubit™ Protein Assay Kit to perform Protein quantification Fluorimetric method

Get tips on using RIPA Lysis Buffer (ProteinSimple) to perform Protein isolation Tissue - Mouse cardiac tissue

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Unstained Protein Ladder, Broad Range Ladder to perform Protein Ladder Unstained

Get tips on using SuperSignal™ Molecular Weight Protein Ladder to perform Protein Ladder Unstained

Get tips on using Pierce™ Unstained Protein MW Marker to perform Protein Ladder Unstained