siRNA / miRNA gene silencing Rat Schwann cells

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Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using ROS-ID® Total ROS detection kit to perform ROS assay cell type - human umbelical vein endothelial cells (HUVEC)

Products Enzo Life Sciences ROS-ID® Total ROS detection kit

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - T-cells Mouse (CD4+ and CD8+)

Products BD Biosciences PE Annexin V Apoptosis Detection Kit I

Get tips on using Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12

Get tips on using ApopTag® Fluorescein In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - Islets of langerhans (Beta cells)

Products Millipore ApopTag® Fluorescein In Situ Apoptosis Detection Kit

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media NCH421K cells primary glioma

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

Get tips on using The Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit to perform Autophagy assay cell type - Human osteosarcoma cancer cells

Products Thermo Fisher Scientific The Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit

Get tips on using FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme to perform Apoptosis assay cell type - Human endometrial stromal cells

Products Millipore FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Get tips on using pwPICZalpha-DT390-bi-pIL-2-Gly to perform Protein Expression Eukaryotic cells - P. pastoris Porcine IL-2 fusion toxins

Products Zhirui Wang, Transplantation Biology Research Center, Massachuse pwPICZalpha-DT390-bi-pIL-2-Gly

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