siRNA / miRNA gene silencing Human CRL-5915

Get tips on using pBru-prochymosin to perform Protein Expression Prokaryotic cells - E. coli calf prochymosin

Get tips on using pET24b-prochymosin to perform Protein Expression Prokaryotic cells - E. coli calf prochymosin

Get tips on using CIRCLEGROW AGAR (POWDER) to perform Bacterial cell culture media Escherichia coli

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media CML T1

Get tips on using CAPNS1ΔGR/pACpET to perform Protein Expression Prokaryotic cells - E. coli rh μ‐calpain

Get tips on using CAPNS1/pACpET to perform Protein Expression Prokaryotic cells - E. coli rh μ‐calpain

Get tips on using IBI’s Todd-Hewitt Broth to perform Bacterial cell culture media Escherichia coli

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using pIEX-4-Crypα-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - S. frugiperda mMBP

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media CML T1