rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform 3D Cell Culture Media Mouse endometrium organoids

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2

Get tips on using Anti-Human CD284 (TLR4) to perform Flowcytometry TLR4 (CD284) - Mouse / IgG1, kappa Human Brilliant violet 421

Get tips on using FlexiTube GeneSolution GS27279 for Tnfrsf12a to perform siRNA / miRNA gene silencing Mouse - B16-BL6 FN14/Tnfrsf12a

Get tips on using Anti-Integrin β1 Antibody, clone MB1.2 to perform Flow cytometry Anti-bodies Mouse - CD29/β1-Integrin

Get tips on using QuikChange Lightning Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion L929 ICP6

Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Mouse - Deletion 3T3-L1 fmnl 2/3

Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - L929 capsid protein

Get tips on using CD14 Antibody, Pacific Orange™ conjugate to perform Flowcytometry CD14 - Mouse / IgG2a Human Pacific Orange™