siRNA / miRNA gene silencing Human WI-38

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using SENSE mRNA-Seq Library Prep Kit V2 to perform RNA sequencing Rat - Hippocampal tissue

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Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Get tips on using Wide-Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Thioglycolate Broth with Resazurine to perform Bacterial cell culture media Clostridium tetani

Get tips on using truChIP Chromatin Shearing Kit with Formaldehyde to perform ChIP Mouse - Brain

Get tips on using LB Broth with agar (Lennox) to perform Bacterial cell culture media Vibrio cholera

Get tips on using LB Broth with agar (Lennox) to perform Bacterial cell culture media Salmonella Typhi