rna-isolation-purification-cells-immortalized-cos-7

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Goblet cells

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - SAE cells

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - K562 cells

Get tips on using siGENOME Rat Nrp1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrp1

Get tips on using SEC-GFP-pHis1522 to perform Protein Expression Prokaryotic cells - Brevibacillus choshinensis SP3 α-amylase

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - K562 human leukemia cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using DMEM (Dulbecco's Modified Eagle's medium) to perform Stem cell culture media Cord blood-derived endothelial cells(hCBiPS2)

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Human - Endometrial stromal cells Target preparation kit (Amplification + Hybridization + control)