siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - CHO-K1

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - SH-SY5Y

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse primordial germ cells

Get tips on using Hydroxymethyl Collector™ Kit to perform DNA methylation profiling Whole genome profiling - mouse primordial germ cells

Get tips on using QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric to perform Cell migration / Invasion cell type - PANC-1

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Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Renal carcinoma cell line Caki-1

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line DLD-1

Get tips on using PE-Cy™7 Rat Anti-Mouse CD31 to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1