ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α -NA- Human

Get tips on using Mouse Chitinase 3-like 1 Quantikine ELISA Kit to perform ELISA Mouse - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Get tips on using Rat Retinol binding protein 4,RBP-4 ELISA Kit to perform ELISA Rat - RBP4

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Rat - IGF-I

Get tips on using Rat CRP/C Reactive Protein PicoKine™ ELISA Kit to perform ELISA Rat - C-Reactive Protein/CRP

Get tips on using NFκB p65 Antibody (F-6): sc-8008 to perform Immunohistochemistry Mouse - NFκB / p65

Get tips on using cytochrome c Antibody (A-8): sc-13156 to perform Western blotting Cytochrome C

Get tips on using Gibco™Essential 8™ Medium to perform Stem cell Differentiation media hPSC or hiPSCS differentiation into myogenic cells

Get tips on using Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit to perform ELISA Mouse - C-Reactive Protein/CRP

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.