Get tips on using Culture-Insert 4 Well in µ-Dish 35 mm, high to perform Cell migration / Invasion cell type - MCF7
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized Rael
Get tips on using Quick-RNA Microprep Kit to perform RNA isolation / purification Cells - immortalized Raji
Get tips on using ApopTag® Peroxidase In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - FaDu human squamous cell carcinoma
RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
Get tips on using LC3B antibody to perform Autophagy assay cell type - RT4
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Muscle
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Kidney
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Brain
Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Bone
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