Protein Expression Eukaryotic cells W. anomalus

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - FTC236

Get tips on using 3 µm Chemotaxis Assays, 96-Well Format to perform Cell migration / Invasion cell type - Jurkat

Get tips on using 5 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - A549

Get tips on using 8 µm Chemotaxis Assays, 96-Well Format to perform Cell migration / Invasion cell type - MCF-10A

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - PANC-1

Get tips on using Corning® BioCoat™ Tumor Invasion 24-well Plate to perform Cell migration / Invasion cell type - SaOS-2

Get tips on using Culture-Insert 4 Well in µ-Dish 35 mm, high to perform Cell migration / Invasion cell type - MCF7

Get tips on using SNP Type™ 96.96 Genotyping Reagent Kit with Control Line Fluid—10 IFCs to perform Cell line authentication Human prostatic cancer cell line DU145

Get tips on using SNP Type™ 96.96 Genotyping Reagent Kit with Control Line Fluid—10 IFCs to perform Cell line authentication Human prostatic cancer cell line PC3

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.