siRNA / miRNA gene silencing Human COV-434

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A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

Cellular assays Angiogenesis assay mouse spleen-derived EPCs

RNA mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Get tips on using TurboCapture 96 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Products Qiagen TurboCapture 96 mRNA Kit (5)

Get tips on using TurboCapture 384 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Products Qiagen TurboCapture 384 mRNA Kit (5)

Get tips on using Oligotex mRNA Midi Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Products Qiagen Oligotex mRNA Midi Kit (12)

Get tips on using Oligotex mRNA Mini Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification polyA+ mRNA

Products Qiagen Oligotex mRNA Mini Kit (12)

Get tips on using MagAttract Direct mRNA M48 Kit (192) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Products Qiagen MagAttract Direct mRNA M48 Kit (192)

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines Renal cortical tubule epithelial cells

Get tips on using PCR SuperMix to perform PCR Conventional / Qualitative PCR - mammalian DNA

Products Thermo Fisher Scientific PCR SuperMix

Get tips on using DreamTaq DNA Polymerases to perform PCR Conventional / Qualitative PCR - bacterial DNA

Products Thermo Fisher Scientific DreamTaq DNA Polymerases

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