CRISPR Rat Deletion INS-1 832/13

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized VCaP

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U87

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Get tips on using siRNA ICAM1 to perform siRNA / miRNA gene silencing Human - HT-1080 ICAM1

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Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.