Cell Isolation

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized U-251

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized Saos-2

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized K-562

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized HT-29

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A-172

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.