Protein expression and purification Bacteria Origami™ B (DE3)

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)

Get tips on using BenchMark™ Protein Ladder to perform Protein Ladder Unstained

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Get tips on using CelLytic™ M to perform Protein isolation Bacteria - Staphylococcus aureus

Get tips on using BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector to perform shRNA gene silencing Mouse - P19 Foxm1

Get tips on using TRI Reagent™ Solution to perform Protein isolation Bacteria - Vibrio cholerae

Get tips on using EZQ™ Protein Quantitation Kit to perform Protein quantification Tissue - mouse brain

Get tips on using TRIzol™ Max™ Bacterial RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Get tips on using PageRuler™ Unstained Protein Ladder to perform Protein Ladder Unstained