RNA isolation / purification Cells Cancer cell lines

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Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - T-cells Mouse (CD4+ and CD8+)

Products BD Biosciences PE Annexin V Apoptosis Detection Kit I

Get tips on using Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12

Get tips on using ApopTag® Fluorescein In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - Islets of langerhans (Beta cells)

Products Millipore ApopTag® Fluorescein In Situ Apoptosis Detection Kit

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media NCH421K cells primary glioma

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

Get tips on using FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme to perform Apoptosis assay cell type - Human endometrial stromal cells

Products Millipore FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

I have extracted RNA from brain tissue but my RNA concentrations are as low as 5ng/ml with my highest being around 80ng/ml. Will I be able to perform cDNA conversion with concentrations as low as these?

Discussions cDNA conversion with low concentrations

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media hESCs or iPSCs differentiation into ovarian follicle/granulosa cells

Products Thermo Fisher Scientific Gibco™DMEM/F-12

Get tips on using Gibco™KnockOut™ DMEM to perform Stem cell Differentiation media hESCs or iPSCs differentiation into ovarian follicle/granulosa cells

Products Thermo Fisher Scientific Gibco™KnockOut™ DMEM

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Products Promega DeadEnd™ Colorimetric TUNEL System

Get tips on using Gibco™Advanced DMEM/F-12 to perform Stem cell Differentiation media hPSCs or iPSCs differentiation into Lung progenitor cells

Products Thermo Fisher Scientific Gibco™Advanced DMEM/F-12

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