Protein Expression Eukaryotic cells N. benthamiana

Get tips on using NruI NEB#R0192 to perform Restriction Enzymes NruI / Bsp68I

Get tips on using NOTCH1/activated Notch1 Antibody to perform Immunohistochemistry Mouse - Notch1

Get tips on using Anti-NSE, Clone MIG-N3 to perform Immunohistochemistry Mouse - NSE

Get tips on using Human/Mouse NLRP3/NALP3 Antibody to perform Western blotting NLRP3

Get tips on using Rat NGF/NGF Beta PicoKine™ ELISA Kit to perform ELISA Rat - beta-NGF

Get tips on using Negative control, native pGL4.13 luciferase vector to perform Reporter gene assay luciferase - negative control (luciferase vector)

Get tips on using Silencer® Select Negative Control No 1 siRNA to perform siRNA / miRNA gene silencing Human - siRNA negative control Lipid

Get tips on using Silencer® Select Negative Control No 1 siRNA to perform siRNA / miRNA gene silencing Mouse - siRNA negative control polymer / lipid

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Get tips on using Nrf2 siRNA (r) to perform siRNA / miRNA gene silencing Rat - NRCM Nrf2