siRNA / miRNA gene silencing Rat RMC-1

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MTD-1A

Products Qiagen RNeasy Mini Kit

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Hepa-1c1c7

Products Qiagen RNeasy Mini Kit

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized HT-1080

Products Qiagen RNeasy Mini Kit

Get tips on using BECN1 Antibody (H-300) to perform Autophagy assay cell type - INS-1E

Products Santa Cruz Biotechnology BECN1 Antibody (H-300)

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - INS-1E

Products Sigma-Aldrich Autophagy Inhibitor, 3-MA

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized C3H-10T1/2

Products Qiagen RNeasy Mini Kit

Get tips on using JNK1 Antibody (F-3) to perform Autophagy assay cell type - PC-12

Products Santa Cruz Biotechnology JNK1 Antibody (F-3)

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - HCT-116

Products BD Biosciences PI/RNase Staining Buffer

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - MCF 10A

Products BD Biosciences APC BrdU Flow Kit

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

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