PCR

Get tips on using pCas-Guide-EF1a-GFP to perform CRISPR Mouse - Deletion αT3 Stim2

Get tips on using pcDNA-mC/EBPb to perform CRISPR Mouse - Activation 3T3-L1 C/EBPβ

Get tips on using TRIzol Reagent to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Get tips on using PyroMark CpG Assays to perform DNA methylation profiling Whole genome profiling - human whole blood

Get tips on using pIRES2-EGFP-PBD-1 to perform Protein Expression Prokaryotic cells - E. coli PBD1-EGFP

Get tips on using PrimaPure Porcine Endothelial Cells Growth Medium to perform Mammalian cell culture media PAOEC

Get tips on using DNeasy UltraClean Microbial Kit to perform DNA isolation / purification Yeast - Candida parapsilosis

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Get tips on using FOXP3 Monoclonal Antibody (PCH101), PE, eBioscience™ to perform Flow cytometry Anti-bodies Human - FOXP3

Get tips on using CD25 Monoclonal Antibody (PC61.5), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD25