rna-isolation-purification-cells-primary-human-carotid-artery-endothelial-cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human epidermal keratinocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human cardiac fibroblasts

Get tips on using Xfect™ Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary porcine primary chondrocytes

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes