siRNA / miRNA gene silencing Mouse RGC-5

Get tips on using Accell Mouse Nrep (27528) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 P311/Nrep

Get tips on using ON-TARGET plus Mouse Becn1 (56208) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 4T1 BECN-1

Get tips on using ON-TARGETplus Mouse Sphk1 (20698) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MC3T3-E1 SphK1

Get tips on using ON-TARGETplus Mouse Fdps (110196) siRNA - Individual, to perform siRNA / miRNA gene silencing Mouse - MC3T3-E1 Fdps

Get tips on using ON-TARGETplus Mouse Mprip (26936) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Mprip

Get tips on using ON-TARGETplus Mouse Casp8 (12370) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Casp8

Get tips on using ON-TARGETplus Mouse Myb (17863) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - NIH-3T3 Myb

Get tips on using ON-TARGETplus Mouse Eif2ak3 (13666) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - CT26 Perk/Eif2ak3

Get tips on using ON-TARGETplus Mouse Casp8 (12370) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - CT26 caspase-8

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.