DNA isolation / purification Cells Primary cells

Get tips on using MagniSort™ Human pan-Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using Hot StarTaq Master Mix Kit (2500 U) to perform PCR Hot start PCR - Mammalian DNA

Get tips on using QuantiTect SYBR Green RT-PCR Kit (1000) to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using EpiTect MethyLight PCR +ROX Vial Kit (200) to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Mouse - NIH 3T3

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Human - Hep G2

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Human - SH-SY5Y

Get tips on using GenLadder 50bp (ready-to-use) with dye Orange G to perform DNA Ladder 50 bp

Get tips on using pCEP4 Mammalian Expression Vector to perform Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.