siRNA / miRNA gene silencing Rat Retinal stem cells

Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-361

Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using Bac-to-Bac™ Baculovirus Expression System to perform Protein expression and purification Insect cells - Sf9 Drosha

Get tips on using PRO-PREP™ Protein Extraction Solution (C/T) to perform Protein isolation Mammalian cells - Mouse Epididymal fat

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - human metastatic melanoma cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Islets of langerhans (Beta cells)

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - Cancer cell lines Leukemia cancer cell lines KG-1