siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - PANC-1

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - THP 1

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - TPC 1

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HL-1

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - THP 1

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis PANC-1

Get tips on using pCW-LIC to perform Protein expression and purification Bacteria - Escherichia coli Fbxo7

Get tips on using TAGZyme DAPase Enzyme (50 U) to perform Protein tag His-tag removal

Get tips on using GeneJuice® Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines 3T3-L1

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.