Apoptosis assay cell type - Array of apoptotic proteins

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 1 matching solution for this experiment

Proteome Profiler™ Human Apoptosis Array Kit

R&D system, Minneapolis, MN, USA

Protocol tips
T cells were treated with or without CCF52 ganglioside (15μg/ml) for 48hrs. Thereafter, cell lysates were subjected to analysis using the Proteome Profiler human apoptosis antibody array kit from R&D Systems (Minneapolis, MN) according to the manufacturer’s instructions. Arrays were developed with streptavidin-HRP for 30min on a rocking platform shaker. Developed signals were densitized using Image J software, pixel densities were normalized to untreated sample and expressed as mean pixel density.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms