Site Directed Mutagenesis (SDM) Rat Deletion H9C2

Get tips on using Omniscript RT Kit (200) to perform cDNA synthesis Cell lines

Get tips on using Sensiscript RT Kit (200) to perform cDNA synthesis Cell lines

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized 293T

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized VCaP

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U87

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized T98G

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized PNT2

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized LNCaP

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized DU145

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.