PCR Quantitative real-time PCR

Get tips on using pgMAX system-Bgeo to perform Protein Expression Prokaryotic cells - E. coli Bgeo

Get tips on using pHT43-BMP2-D to perform Protein Expression Prokaryotic cells - B. subtilis rhBMP2

Get tips on using pHT43-BMP2-M to perform Protein Expression Prokaryotic cells - B. subtilis rhBMP2

Get tips on using pRNAT-H1.1/Neo to perform shRNA gene silencing Mouse - 4T1 Integrin α6

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Mouse - Ovary

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Human - PBMC

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Human - HUVEC

Get tips on using Mouse proMMP9 ELISA Kit (ab100732) to perform ELISA Mouse - MMP9

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.