siRNA / miRNA gene silencing Human T47-D

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Mouse Decorin ELISA Kit (DCN) (ab155454) to perform ELISA Mouse - Decorin

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media DAOY

Get tips on using DMEM (Dulbecco's Modified Eagle's medium) to perform Mammalian cell culture media DH82

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media DH82

Get tips on using Quantifiler™ Duo DNA Quantification Kit to perform DNA quantification Blood

Get tips on using 200 bp DNA Ladder (Dye Plus) to perform DNA Ladder 200 bp

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media A2780/DDP

Get tips on using EZ DNA Methylation-Direct Kit to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using QIAamp DSP DNA Blood Mini Kit to perform DNA isolation / purification Tissue - blood / plasma