DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HSG cells

Get tips on using HE4 CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Repression HE4

Get tips on using HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5] to perform Immunohistochemistry Human - Hes1

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression lncRNA PVT1

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression lncRNA PVT1

Get tips on using pHR-SFFV-KRAB-dCas9-P2A-mCherry to perform CRISPR Human - Repression MYC

Get tips on using pHR-SFFV-KRAB-dCas9-P2A-mCherry to perform CRISPR Human - Repression GATA1

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.