Protein Expression Prokaryotic cells L. citreum

Get tips on using DMEM/HAM'S F-12 LIQUID MEDIUM WITHOUT L-GLUTAMINE to perform Mammalian cell culture media RBMVEC

Get tips on using ExpiCHO™ Expression System Kit to perform Protein expression and purification Mammalian cells - CHO-K1 SUMO protein

Get tips on using DMEM/Ham's F-12 liquid medium w/o L-Glutamine to perform Stem cell culture media mTSPCS

Get tips on using D-MEM (High Glucose) with L-Glutamine and Phenol Red to perform Stem cell culture media hTrophoblasts

Get tips on using D-MEM (High Glucose) with L-Glutamine and Phenol Red to perform Mammalian cell culture media HLF

Get tips on using DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate to perform Mammalian cell culture media U87

Get tips on using D-MEM (High Glucose) with L-Glutamine and Phenol Red to perform Stem cell culture media Mouse pericytes

Get tips on using pgMAX system-rabbit voltage-dependent calcium channel β2a subunit to perform Protein Expression Prokaryotic cells - E. coli rabbit voltage-dependent calcium channel β2a subunit

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.