Site Directed Mutagenesis (SDM) Human Deletion HEK 293T

Get tips on using ON-TARGETplus Human SLC7A2 (6542) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 SLC7A2

Get tips on using ON-TARGETplus Human SLC4A2 (6522) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 SLC4A2

Get tips on using ON-TARGETplus Human BEST1 (7439) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 BEST1

Get tips on using ON-TARGETplus Human CLCN3 (1182) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CLCN3

Get tips on using ON-TARGETplus Human ANO1 (55107) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 ANO1

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using siGENOME Human ARAP1 (116985) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa ARAP1

Get tips on using siGENOME Human AP1G1 (164) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa γ1-adaptin/AP1G1

Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Mouse - Deletion ES (embryonic stem) cells Etv2 promoter

Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Mouse - Deletion 3T3-L1 SWELL1