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shRNA gene silencing

- Found 2843 results

RNA siRNA / miRNA gene silencing Rat Astrocytes HMG-1

RNA siRNA / miRNA gene silencing Rat RMC-1 Cx43

RNA siRNA / miRNA gene silencing Rat RGC-5 IGF1R

RNA siRNA / miRNA gene silencing Rat H19-7 EpoR

RNA siRNA / miRNA gene silencing Human CAL-27 ITGB5

RNA siRNA / miRNA gene silencing Human HT-1080 RELA

Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Products Qiagen Attractene Transfection Reagent

Get tips on using pSilencer 2.1-U6 Hygro to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Beclin 1

Products Thermo Fisher Scientific pSilencer 2.1-U6 Hygro

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

RNA siRNA / miRNA gene silencing Human MCF-7 PRC (PGC-1α–related coactivator)/PPRC1

RNA siRNA / miRNA gene silencing Human siRNA negative control Lipid

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