siRNA / RNAi /miRNA transfection Mouse

Get tips on using ON-TARGETplus Mouse Tead1 (21676) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - C2C12 Tead1

Get tips on using ON-TARGETplus Mouse Sirt2 (64383) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Sirt2

Get tips on using ON-TARGETplus Mouse Bnip3 (12176) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RAW264.7 BNIP3

Get tips on using ON-TARGETplus Mouse Rheb (19744) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Rheb

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using ON-TARGETplus Mouse Sphk1 (20698) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MC3T3-E1 SphK1

Get tips on using ON-TARGETplus Mouse Fdps (110196) siRNA - Individual, to perform siRNA / miRNA gene silencing Mouse - MC3T3-E1 Fdps

Get tips on using ON-TARGETplus Mouse Mprip (26936) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Mprip