shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using PRO-PREP™ Protein Extraction Solution (C/T) to perform Protein isolation Mammalian cells - Mouse Epididymal fat

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - mouse microglia

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

Get tips on using Nitrocef disks to perform Reporter gene assay β-lactamase substrates - HEK 293 & HEK 293T cells

Get tips on using Renilla luciferase vector, pGL4.74 to perform Reporter gene assay luciferase - primary human endometrial stromal cells

Get tips on using Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P) to perform Immunohistochemistry Mouse - TUBB3

Get tips on using Granzyme Monoclonal Antibody (NGZB), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Get tips on using Granzyme B Monoclonal Antibody (NGZB), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Get tips on using CD184 (CXCR4) Monoclonal Antibody (2B11), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD184/CXCR4