Western blot 1,4 β-N-acetyl-D-glucosamine

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There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.