Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using EZ-10 Spin Column PCR Product Purification kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication MDA‐MB‐231 cell line

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb #12741 to perform Autophagy assay cell type - HEK 293

Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - Raw 264.7

Get tips on using 96-Well Cell Invasion Assay, Collagen I to perform Cell migration / Invasion cell type - MDA-MB-231

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Cell cytotoxicity / Proliferation assay cell type - HT-22

Get tips on using ONE-Glo™ Luciferase Assay System to perform Reporter gene assay luciferase - BHK-21 baby hamster kidney cells

Get tips on using pSUPER.retro.neo+gfp vector- Syn G (exon 3) siRNA to perform shRNA gene silencing Mouse - RGC-5 Syn G (Exon 3)