Get tips on using pANC230 (xylD) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylD
Get tips on using pANC223 (xylC) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylC
Get tips on using pANC210 (xylB) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylB
Get tips on using pANC209 (xylA) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylA
Get tips on using Quant-iT™ RNA Assay Kit to perform RNA quantification Fuorimetric - mouse glial cells
Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.
Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA
Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - Spleen cells
Get tips on using pET-28a(+)-g-α13 to perform Protein Expression Prokaryotic cells - E. coli α-13 giardin
Get tips on using GenJet™ In Vitro DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines COS7
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