RNA isolation / purification Bacteria

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Get tips on using Genomic DNA MiniSpin: Bacteria to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.