rna-isolation-purification-cells-primary-canine-peripheral-blood-mononuclear-cells

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Get tips on using DNase Max Kit (50) to perform Removal of contamination in RNA DNA contamination

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR

Get tips on using ScriptSeq Complete Kit (Human/Mouse/Rat) to perform RNA sequencing Mouse - Bone marrow-derived macrophages (BMDMs)

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR - fibroblast